EVALUATION OF CHEMICAL COMPOSITION AND IN VITRO ANTICHOLINESTERASIC ACTIVITY OF ESSENTIAL OILS FROM HYMENAEA CANGACEIRA AND EUGENIA GRACILLIMA

• DOI: 10.22533/at.ed.1592262227059

• Palavras-chave: Alzheimer's disease. Acetylcholinesterase. Butyrylcholinesterase. Enzyme Inhibition. Natural products.

• Keywords: Alzheimer's disease. Acetylcholinesterase. Butyrylcholinesterase. Enzyme Inhibition. Natural products.

• Abstract:

  Plants have been manipulated for therapeutic purposes with the aim of preventing, treating and curing pathologies. Inhibition of cholinesterases contributes to increased bioavailability of the neurotransmitter acetylcholine in the synaptic cleft. The therapy for Alzheimer's disease (AD) consists of inhibiting cholinesterases, and natural products are rich sources of these inhibitors. This study aimed to evaluate the chemical composition and in vitro anticholinesterase activity of essential oils from Hymenaea cangaceira (OEHc) and Eugenia gracilima (OEEg). The essential oils were obtained by hydrodistillation and evaluated for composition in a gas chromatograph, coupled to a mass spectrometer (GC-MS) and flame ionization detector (GC-DIC). Cholinesterase inhibition was performed in in vitro colorimetric assays, using acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), the results being expressed in µg/mL of the concentration required for IC50. Neostigmine was used as a reference standard. The OEHc presented 15 compounds and the OEEg 42 compounds. In the evaluation of AChE inhibition, the OEHc presented an IC50 of 0.015µg/mL, being statistically lower than the OEEg (IC50 of 18.30µg/mL). The OEHc against the BChE enzyme presented an IC50 of 0.041µg/mL, being lower than the OEEg, which obtained an IC50 of 8.67µg/mL. OEHc obtained greater inhibitory activity on AChE and BChE when compared to OEEg. The results suggest that OEHc has potential for use in the clinical treatment of neurodegenerative diseases, since it decreases AChE and BChE activity in vitro.

• Número de páginas: 15