EVALUATION OF BACTERIA ISOLATED FROM PEANUT CULTURE FOR PHOSPHATE SOLUBILIZATION AND THEIR PHOSPHATASE ENZYMES
EVALUATION OF BACTERIA ISOLATED FROM PEANUT CULTURE FOR PHOSPHATE SOLUBILIZATION AND THEIR PHOSPHATASE ENZYMES
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DOI: https://doi.org/10.22533/at.ed.1813622607053
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Palavras-chave: Arachis hipogaea L.; fosfatase ácida; fósforo; solo
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Keywords: Arachis hypogaea L.; acid phosphatase; phosphorus; soil
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Abstract: Peanut (Arachis hypogaea L.) is a crop of great economic relevance in Brazil, whose productivity is often limited by the low availability of phosphorus (P) in soils. This study aimed to analyze the phosphate-solubilizing capacity of bacteria collected from peanut-cultivated soils, as well as to investigate their phosphatase activity. The potential of two bacterial strains (Burkholderia and Paraburkholderia) isolated from the rhizosphere to solubilize phosphates was assessed, and the kinetic parameters of acid phosphatase produced by these microorganisms were characterized. The bacteria were grown in medium containing different phosphate concentrations (0.1 mM, 0.5 mM, and 1.0 mM), and enzymatic activity was analyzed as a function of pH, reaction time, and the presence of ions (Zn²⁺ and Mg²⁺). Results demonstrated that acidic pH conditions and low phosphate concentrations favored both bacterial growth and phosphatase activity, with the enzyme being predominantly localized in the membrane fraction. The presence of Zn²⁺ and Mg²⁺ positively influenced enzymatic activity, suggesting their role as cofactors. It is concluded that the studied strains have significant potential to increase phosphorus availability in peanut-cultivated soils, offering a sustainable alternative to improve soil fertility and crop productivity, particularly in areas with phosphorus deficiency.
- Luiz Flávio José dos Santos
- JAQUELINE DEL GROSSI
- ISADORA MARIA ZIVIANI FERNANDES
- ANNA CAROLINA DE OLIVEIRA SOUZA