"Evaluation of Hematological and Biochemical Blood Parameters in Guinea Pigs (Cavia porcellus) supplemented with Nanoemulsions of Essential Oils".

• DOI: https://doi.org/10.22533/at.ed.973542512034

• Palavras-chave: nanoemulsion, essential oils, hematological parameters, blood biochemistry, guinea pig.

• Keywords: nanoemulsion, essential oils, hematological parameters, blood biochemistry, guinea pig.

• Abstract:

  The objective of this study was to evaluate the hematological and biochemical blood parameters of guinea pigs (Cavia porcellus) supplemented with nanoemulsions of essential oils (EO) of chincho and oregano. Fifty hybrid male guinea pigs of the Peruvian and Andean breeds (average weight 280 g) were randomly distributed in 5 experimental groups, with 10 replicates each: T1 (Control Group), T2 (base diet + APC: 200 ppm bacitracin zinc), T3 (base diet + 200 ppm chincho EO nanoemulsion), T4 (base diet + 200 ppm oregano EO nanoemulsion) and T5 (base diet + 200 ppm chincho and oregano EO nanoemulsion). Venous blood samples were taken during the benefit of the animals, 49 days after the beginning of the study. The hematological parameters evaluated were primary and secondary erythrocyte indices, absolute and relative leukocyte count. In blood biochemistry, glucose, triglycerides, cholesterol and total proteins were measured. Analysis of variance (ANOVA) and Tukey's test were applied. In the red series, T2 presented a significant difference (p<0.05) compared to T1 in MCV (Mean Corpuscular Volume). Within the leukocyte series, no significant difference was found; however, T1 exceeded the reference value in monocytes. In addition, a significant reduction (p<0.05) in glucose levels was observed in the T3 group in contrast to T1. Globulins of T3, T4 and T5 were significantly (p<0.05) higher compared to T1. However, the values remained within the accepted limits according to reference standards. It is concluded that adding oregano and chincho EO nanoemulsions to the guinea pig diet does not affect blood parameters and could be a natural alternative to the use of APC in animal feed.