EFFECT OF THE AQUEOUS EXTRACT OF CALEA URTICIFOLIA (Mill) DC IN ADIPOCYTES 3T3-L1 INSULIN RESISTANCE.

• DOI: 10.22533/at.ed.8133102307115

• Palavras-chave: -

• Keywords: Calea urticifolia, insulin resistance, glucose, low-grade inflammation.

• Abstract:

  Insulin resistance (IR) is a metabolic condition linked to various comorbidities, including type 2 diabetes mellitus (DM-2), fatty liver, atherosclerosis, and hypertension. Several mechanisms contribute to IR, such as insulin receptor insensitivity, dysfunction in glucose transporter (GLUT-4) expression and activity, chronic inflammation marked by elevated proinflammatory cytokines like tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6), and oxidative stress, all of which impede glucose utilization in differentiated adipocytes. Calea urticifolia (Cu) is traditionally employed for enhancing glucose homeostasis; thus, the biological effects must be evaluated. Mature adipocytes (3T3-L1 line) with induced IR were used to assess the hypoglycemic impact of Cu´s aqueous Cu-Extract (Cu-Extract) Glucose internalization assays in IR adipocytes, exposed to different concentrations of the Cu-Extract (5.5, 11, and 55µg/mL), Metformin (7µg/mL), and a combination of both, were conducted to determine the optimal concentration for GLUT-4 and AKT expression. The Cu-Extract demonstrated a hypoglycemic effect by facilitating glucose uptake through both insulin-dependent and insulin-independent processes, involving AMPK and AKT phosphorylation.