Detection and molecular characterization of Bovine papillomavirus subtypes in cattle from two Colombian regions

• DOI: 10.22533/at.ed.813322308026

• Palavras-chave: -

• Keywords: Bovine papillomavirus (BPV), BPV2, BPV3, Colombia, papillomatous lesions

• Abstract:

  Bovine papillomaviruses (BPV) are oncogenic viruses developing benign and malignant
  hyperplastic lesions of cutaneous, mucosal, upper gastrointestinal epithelia, and bladder in cattle. In
  this study, we report the subtypes of BPV isolated from Colombian cattle and their molecular
  characterization. Sixteen biopsies were sampled from papillomatous lesions in two Colombian
  cattle farming regions. Detection and subtype identification were done by amplifying a 450 bp
  fragment of the L1 gene using the consensus primers MY09/MY11, followed by Sanger
  sequencing. Fourteen out of 16 samples tested positive for BPV. Twelve of these 14 samples were
  clustering with the BPV2 subtype and two with BPV3. The BPV phylogeny analysis showed that
  the BPV2 Colombian isolated clusters according to the geographical areas of origin. Furthermore,
  the L1 amino acid substitution most often found in the fragment analyzed in the BPV-2 Colombian
  isolates was leucine to valine at 386 (Leu386Val). As far as we know, this is Colombia's first report
  of BPV subtypes; BPV2 was the most common strain found, and BPV1 was not identified.