DNA detection from Schistosoma mansoni by loop-mediated isothermal amplification: Identification of infected molluscs in the prepatent period

• DOI: 10.22533/at.ed.159392303027

• Palavras-chave: Schistosoma mansoni; Biomphalaria glabrata; Loop-mediated- Isothermal Amplification

• Keywords: Schistosoma mansoni; Biomphalaria glabrata; Loop-mediated- Isothermal Amplification

• Abstract:

  Schistosomiasis is a neglected tropical disease, which infects about 240 million individuals worldwide. It is a parasitic infection transmitted by water, whose intermediate host is a mollusc of the genus: Biomphalaria. The aim of this study was to standardize the detection of DNA from Schistosoma mansoni by means of the loop-mediated isothermal amplification (LAMP) technique in samples of primary sporocysts, obtained from the laboratory cycle of Biomphalaria glabrata, strain Ourinhos, infected by S. mansoni, strain BH, at the Laboratory of Immunopathology of Schistosomiasis (LIM-06), Faculty of Medicine, ‘’Universidade de São Paulo’’ (FMUSP), São Paulo, Brazil. Results: We advanced in the elaboration of the LAMP technique protocol. The identification of molluscs infected by S.mansoni in the pre-patent period may contribute to surveillance actions.