Degradation of the compound 4-Nitrophenol, by means of an electrochemical biosensor of purified Laccase Enzyme "Myceliophthora Thermophila"

• DOI: 10.22533/at.ed.3173142302053

• Palavras-chave: -

• Keywords: Myceliophthota Thermophila, 4-Nitrophenol, Electrochemical Biosensor

• Abstract:

  In the last decades, the contamination of the terrestrial ecosystem and aquifers has increased due
  to the indiscriminate use of a group of highly polluting and difficult to degrade organic
  compounds, such as phenolic compounds used at industrial level (paints, antibiotics, etc.); these
  compounds have a molecular structure containing at least one phenol group (aromatic ring linked
  to a hydroxyl functional group, so they have been classified as primary pollutants to be
  degraded). In this work we present the results obtained from the purification of the Lacase
  Enzyme "Myceliophthota Thermophila", by Fast Protein Liquid Chromatography (FPLC) and
  electrophoresis, used in the design of a biosensor capable of degrading the compound 4-
  Nitrophenol (4-NF), the electrocatalytic efficiency of this was followed by cyclic voltammetry
  obtaining a good response for the degradation of 4-NF obtaining as detection limit of 7. 189 ppm,
  limit of quantification of 25.165 ppm with a Jo exchange current density of 76.2x10 -3 µA/cm -2 , by
  UV-Vis spectrophotometry a linear trend was obtained with a slope of -0.04 AU/days and
  correlation coefficient of 0.9954. By means of this information it was corroborated that the
  biosensor is a good candidate in the degradation of 4-NF as a function of time, the FT-IR
  spectroscopic technique shows a structural change of the enzyme Lacase when it is used in the
  degradation of 4-NF.