CYTOTOXICITY EFFECTS AND CHEMOTHERAPY IMPROVEMENT OF COCOA AGAINST CHRONIC MYELOCYTIC LEUKEMIA CELL LINE (K562)
CYTOTOXICITY EFFECTS AND CHEMOTHERAPY IMPROVEMENT OF COCOA AGAINST CHRONIC MYELOCYTIC LEUKEMIA CELL LINE (K562)
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DOI: https://doi.org/10.22533/at.ed.15947924210810
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Palavras-chave: -
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Keywords: in vitro, Theobroma cacao L., leukemia, ATRA, arsenic trioxide, anticancer
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Abstract: This study aimed to analyze the anticarcinogenic activity and in vitro response to chemotherapy of cocoa in chronic myeloid leukemia (CML) cell line (K562). Cells were exposed to different concentrations of cocoa (30; 100; 500; 750; 1000; 1500 and 2000 μg/mL) isolated and associated with ATRA and arsenic trioxide during 24 and 72 hours. Our results indicate that cocoa showed selective cytotoxic action on K562 cells at all concentrations tested in 72 hours. Moreover, cocoa raised oxidative stress by increasing levels of total levels of ROS, nitric oxide, and superoxide. Also, cocoa improved the action of chemotherapy, by reducing cell viability and proliferation. Thus, the results highlight cocoa as a potent anticancer agent, with an excellent selective profile to be used adjuvant therapies for CML.
- aline costa lopes
- Camila Medianeira da Silva D'Ávila
- Tallys de Oliveira Mendes
- Isadora Cassel Livinalli
- Ana Clara Zanini Bertoncelli
- Alice Kluge Borba
- Altevir Rossato Viana
- Luciana Maria Fontanari Krause
- Francine Carla Cadoná
