BIOLOGICAL CONTROL in vitro of Colletotrichum sp. ISOLATED FROM ROÑA SYNDROME IN AVOCADO Persea americana Mill. IN MICHOACAN
Revisiones fitosanitarias en diferentes huertas de aguacate en zonas agroecológicas de Michoacán revelan diversidad de síntomas atribuidos a Colletotrichum spp. El objetivo de esta investigación fue aislar los patógenos asociados al síndrome de roña en aguacate, comprobar la patogénesis de los mismos, así como realizar un control biológico in vitro. Se recolectaron muestras de frutos de aguacate con el síndrome de roña en los municipios de San Juan Nuevo, Los Reyes y Uruapan. Se aisló el patógeno, se realizaron las pruebas de patogenicidad y el control biológico in vitro se llevó a cabo en base a cuatro tratamientos Baktillis (Bacillus subtilis) 1 mL/L, Natucontrol (Trichoderma harzianum), 0.3 g/L, Labrador (toxinas de T. harzianum) 0.024 g/L y Bactiva (Bacillus spp.) 0.16 g/L, y un testigo absoluto, con diseño completamente al azar. Se determinó la presencia de Colletotrichum gloeosporioides., en base a los atributos morfológicos. En los frutos inoculados se produjeron síntomas de corchosis, que no fueron similares a los síntomas que presentaban los frutos de los cuales se aisló el patógeno. Por lo cual Colletotrichum gloeosporioides. también puede provocar síndrome de roña, pero no es el responsable de la roña típica o mancha purpura en aguacate. El producto que tuvo un mayor control fue Natucontrol (T. harzianum).
BIOLOGICAL CONTROL in vitro of Colletotrichum sp. ISOLATED FROM ROÑA SYNDROME IN AVOCADO Persea americana Mill. IN MICHOACAN
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DOI: 10.22533/at.ed.813332327035
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Palavras-chave: Productos biológicos, Hongos, Colletotrichum spp.
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Keywords: Biological products, Mushrooms, Colletotrichum spp.
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Abstract:
Phytosanitary reviews in different avocado orchards in agroecological zones of Michoacán reveal diversity of symptoms attributed to Colletotrichum spp. The objective of this research was to isolate the pathogens associated with avocado scab syndrome, verify their pathogenesis, as well as carry out an in vitro biological control. Samples of avocado fruits with scab syndrome were collected in the municipalities ofSan Juan Nuevo, Los Reyes and Uruapan. The pathogen was isolated, pathogenicity tests were performed, and in vitro biological control was carried out based on four treatments:Baktillis (Bacillus subtilis) 1 mL/L, Natucontrol (Trichoderma harzianum), 0.3 g/L, Labrador (toxins of T. harzianum) 0.024 g/L and Bactiva (Bacillus spp.) 0.16 g/L, and an absolute Witness, with a completely random design. It was determined the presence of Colletotrichum gloeosporioides., based on morphological attributes. Corchosis symptoms were produced in the inoculated fruits, which were not similar to the symptoms presented by the fruits from which the pathogen was isolated. In which, the Colletotrichum gloeosporioides, it can also cause scab syndrome, but it is not responsible for the typical scab or purple spot on avocado. The product that had greater control was Natucontrol (T. harzianum).
- José Luciano Morales García
- María Elena Salto García
- Karina Lizeth Morales Montelongo
- Edna Esquivel Miguel
