Diagnostic concordance of hematoxylin-eosin and Giemsa staining of gastric biopsies with chemiluminescent immunoassay for stool antigen detection to diagnose Helicobacter pylori infection.
Diagnostic concordance of hematoxylin-eosin and Giemsa staining of gastric biopsies with chemiluminescent immunoassay for stool antigen detection to diagnose Helicobacter pylori infection.
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DOI: https://doi.org/10.22533/at.ed.813472416105
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Palavras-chave: H. pylori, concordancia, inmunoensayo quimioluminiscente, Giemsa, hematoxilina-eosina
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Keywords: H. pylori, concordance, chemiluminescent immunoassay, Giemsa, hematoxylin-eosin.
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Abstract:
Introduction. Identification of Helicobacter pylori is very important for timely treatment. Objective. To determine the level of diagnostic agreement between hematoxylin-eosin and Giemsa staining of gastric biopsies with chemiluminescent immunoassay for detection of antigen in stool to diagnose Helicobacter pylori infection. Methodology. Retrospective observational descriptive study. Statistical analysis was performed using Jamovi 2.3.28 statistical software. Cohen's kappa index was applied to determine the diagnostic agreement between hematoxylin-eosin staining and Giemsa staining with chemiluminescent immunoassay for detection of Helicobacter pylori antigen in stool. Results. The diagnostic agreement when comparing the hematoxylin-eosin stain with the chemiluminescent immunoassay resulted in an acceptable level of agreement by obtaining a value of 0.40 in Cohen's kappa index; the diagnostic agreement when comparing the Giemsa stain with the chemiluminescent immunoassay resulted in a moderate level of agreement by obtaining a value of 0.45 in Cohen's kappa index. Conclusion. Giemsa staining has a better performance; however, since there is no gold standard, it is necessary to evaluate the performance of an additional methodology based on clinical criteria for an accurate diagnosis.
- Jimmy Edy Rolando Godoy Hernández
- Jessenia Sabrina Navas Castillo
- Juan Pablo de León Fajardo
- Gretel Andrea Lemus Arias
- Pablo Alfonso Tzorín Velásquez