CONCENTRATION AND RESISTANCE TO ANTIBIOTICS OF CULTURAL BACTERIA ISOLATED FROM BIOEROSOLS IN THE CLASSROOM

• DOI: 10.22533/at.ed.15939523161110

• Palavras-chave: Occupational exposure to bio-aerosols has been associated with various health effects, as they can contain a wide variety of pathogenic microorganisms, such as bacteria, viruses, fungi and parasites that can pose a threat to humans and animals. In this sense, the purpose of this research is to evaluate the presence and resistance to antibiotics of potentially pathogenic cultivable bacteria in classroom bio-aerosols before, during and after the development of teaching activities. Sample collection was carried out by exposing Petri dishes containing agar media: Blood Agar, Mac Conckey and Baird Parker. Each plate containing sterile culture media will be opened for 30 minutes at a height of 1.5 meters from the floor, arranged in the central part of the room. The plates were incubated at 37oC for 24-48h. There was an increase in bacterial colony-forming units during the course of the class and a reduction after classes. Samples collected before classes showed: Staphylococcus aureus, while during and after the development of classes the following were isolated: Staphylococcus aureus, S. epidemidis, Streptococcus sp, Escherichia coli. All bacteria showed resistance to several antibiotics.

• Keywords: Occupational exposure to bio-aerosols has been associated with various health effects, as they can contain a wide variety of pathogenic microorganisms, such as bacteria, viruses, fungi and parasites that can pose a threat to humans and animals. In this sense, the purpose of this research is to evaluate the presence and resistance to antibiotics of potentially pathogenic cultivable bacteria in classroom bio-aerosols before, during and after the development of teaching activities. Sample collection was carried out by exposing Petri dishes containing agar media: Blood Agar, Mac Conckey and Baird Parker. Each plate containing sterile culture media will be opened for 30 minutes at a height of 1.5 meters from the floor, arranged in the central part of the room. The plates were incubated at 37oC for 24-48h. There was an increase in bacterial colony-forming units during the course of the class and a reduction after classes. Samples collected before classes showed: Staphylococcus aureus, while during and after the development of classes the following were isolated: Staphylococcus aureus, S. epidemidis, Streptococcus sp, Escherichia coli. All bacteria showed resistance to several antibiotics.

• Abstract:

  Occupational exposure to bio-aerosols has been associated with various health effects, as they can contain a wide variety of pathogenic microorganisms, such as bacteria, viruses, fungi and parasites that can pose a threat to humans and animals. In this sense, the purpose of this research is to evaluate the presence and resistance to antibiotics of potentially pathogenic cultivable bacteria in classroom bio-aerosols before, during and after the development of teaching activities. Sample collection was carried out by exposing Petri dishes containing agar media: Blood Agar, Mac Conckey and Baird Parker. Each plate containing sterile culture media will be opened for 30 minutes at a height of 1.5 meters from the floor, arranged in the central part of the room. The plates were incubated at 37oC for 24-48h. There was an increase in bacterial colony-forming units during the course of the class and a reduction after classes. Samples collected before classes showed: Staphylococcus aureus, while during and after the development of classes the following were isolated: Staphylococcus aureus, S. epidemidis, Streptococcus sp, Escherichia coli. All bacteria showed resistance to several antibiotics.