PARTIAL PHENOTYPIC AND 16S rDNA CHARACTERIZATION OF BACTERIA PROMOTING PLANT DEVELOPMENT
Soil microorganisms are closely related to plants, in particular bacteria can help symbiotically or not. The study of promoter bacteria in plant growth is limited, so this work aims to contribute to the knowledge of some phenotypic and genotypic characteristics of bacterial strains for plant development. Two cryopreserved bacterial strains from the FES-Iztacala UNAM Bacterial Culture Collection were requested for analysis. Strain activation and phenotypic analysis were performed; Colonial morphology, Gram stain, biochemical characterization: Oxidation-Fermentation, Catalase, Oxidase and Indole, amplification and sequencing of the 16S rDNA region for BLAST analysis in GenBank. Additionally, genomic DNA of the AAA1, SRA1 and RRJ1 strains previously characterized by morphology and biochemistry and identified as Pseudomonas sp. The RH8530 strain was identified as Rhizobium tropici by traditional tests and 80% similarity with Sinorhizobium meliloti. Molecular analysis determined inconsistency in their characterization in two genomic DNA of the reference strains.
PARTIAL PHENOTYPIC AND 16S rDNA CHARACTERIZATION OF BACTERIA PROMOTING PLANT DEVELOPMENT
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DOI: 10.22533/at.ed.813312301016
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Palavras-chave: BPCV, 16s rDNA, colección de cultivos bacterianos
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Keywords: BPCV, 16s rDNA, colección de cultivos bacterianos.
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Abstract:
Soil microorganisms are closely related to plants, in particular bacteria can help symbiotically or not. The study of promoter bacteria in plant growth is limited, so this work aims to contribute to the knowledge of some phenotypic and genotypic characteristics of bacterial strains for plant development. Two cryopreserved bacterial strains from the FES-Iztacala UNAM Bacterial Culture Collection were requested for analysis. Strain activation and phenotypic analysis were performed; Colonial morphology, Gram stain, biochemical characterization: Oxidation-Fermentation, Catalase, Oxidase and Indole, amplification and sequencing of the 16S rDNA region for BLAST analysis in GenBank. Additionally, genomic DNA of the AAA1, SRA1 and RRJ1 strains previously characterized by morphology and biochemistry and identified as Pseudomonas sp. The RH8530 strain was identified as Rhizobium tropici by traditional tests and 80% similarity with Sinorhizobium meliloti. Molecular analysis determined inconsistency in their characterization in two genomic DNA of the reference strains.
- Alejandro Cruz Monsalvo Reyes
- Ortiz-Sánchez José Roberto
- Molina-González María Graciela.
