Genotypic characterization of K. pneumoniae and E. coli carbapenemases isolated from pediatric samples at the Hospital General de Enfermedades
Antibiotic resistance is a global problem. Among the priority pathogens defined by the World Health Organization (WHO) are enterobacteria resistant to carbapenems (CRE), which increase hospitalization time, cost and mortality and reduce therapeutic options. The early detection of the type of Carbapenemase allows guiding therapeutic approach, knowing epidemiology, controlling intra-hospital infections and avoiding outbreaks. The objective was to genotypically characterize CRE carbapenemases from pediatric samples from a Third Level Hospital in Guatemala.
The study was descriptive, observational, cross-sectional. Of the patients admitted to the Pediatric area from 12/01/2019 to 12/31/2020, the E.coli and K.pneumoniae strains whose MIC showed resistance to imipenem and/or ertapenem were analyzed. Genotypic identification was performed by amplification of the target genes (blaKPC, blaVIM, blaNDM, blaOXA-48 and blaIMP gene) by real-time polymerase chain reaction (PCR) (GeneXpert® Carba-R). The data was processed with SPSS.
62 strains of K. pneumoniae and 24 strains of E. coli resistant to carbapenems were analyzed. 96.77% and 95.83% respectively presented at leastone type of gene evaluated, the most prevalent was blaNDM (59/60, 98.33% and 22/23, 95.65% respectively). One strain of K. pneumoniae presented blaKPC-type carbapenemase and one strain of E. coli presented blaNDM and blaKPC. No genes for VIM, IMP, or OXA-48 carbapenemases were found. The service with the most isolations was the Intensive Care Unit. More than 33% of CRE were recovered from urine cultures.
The genotypic characterization evidenced the presence of the blaNDM gene in more than 95 % of the CRE isolated from pediatric samples from a Third Level Hospital in Guatemala.
Genotypic characterization of K. pneumoniae and E. coli carbapenemases isolated from pediatric samples at the Hospital General de Enfermedades
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DOI: 10.22533/at.ed.1593382330058
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Palavras-chave: Carbapenemase, gene, antibiotic resistance.
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Keywords: Carbapenemase, gene, antibiotic resistance.
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Abstract:
Antibiotic resistance is a global problem. Among the priority pathogens defined by the World Health Organization (WHO) are enterobacteria resistant to carbapenems (CRE), which increase hospitalization time, cost and mortality and reduce therapeutic options. The early detection of the type of Carbapenemase allows guiding therapeutic approach, knowing epidemiology, controlling intra-hospital infections and avoiding outbreaks. The objective was to genotypically characterize CRE carbapenemases from pediatric samples from a Third Level Hospital in Guatemala.
The study was descriptive, observational, cross-sectional. Of the patients admitted to the Pediatric area from 12/01/2019 to 12/31/2020, the E.coli and K.pneumoniae strains whose MIC showed resistance to imipenem and/or ertapenem were analyzed. Genotypic identification was performed by amplification of the target genes (blaKPC, blaVIM, blaNDM, blaOXA-48 and blaIMP gene) by real-time polymerase chain reaction (PCR) (GeneXpert® Carba-R). The data was processed with SPSS.
62 strains of K. pneumoniae and 24 strains of E. coli resistant to carbapenems were analyzed. 96.77% and 95.83% respectively presented at leastone type of gene evaluated, the most prevalent was blaNDM (59/60, 98.33% and 22/23, 95.65% respectively). One strain of K. pneumoniae presented blaKPC-type carbapenemase and one strain of E. coli presented blaNDM and blaKPC. No genes for VIM, IMP, or OXA-48 carbapenemases were found. The service with the most isolations was the Intensive Care Unit. More than 33% of CRE were recovered from urine cultures.
The genotypic characterization evidenced the presence of the blaNDM gene in more than 95 % of the CRE isolated from pediatric samples from a Third Level Hospital in Guatemala.
- Sara Ester Barillas Aragón
- Roger Arturo Gil Cordón
- Edna Carolina Arévalo Valdez
