Pathogenic capacity of Clavibacter michiganensis subsp. michiganensis isolated from tomato on different solanaceae: tomato (Solanum lycopersicum), potato (Solanum tuberosum), eggplant (Solanum melongena), pepper (Capsicum annuum), tobacco (Nicotiana tabacum) and petunia (Petunia hybrida)

• DOI: 10.22533/at.ed.813382330088

• Palavras-chave: Cmm, Solanáceas, Cáncer bacteriano, Patogenicidad

• Keywords: Cmm, Solanaceae, Bacterial cancer, Pathogenicity

• Abstract:

  Clavibacter michiganensis subsp. michiganensis (Cmm), is a plant pathogenic actinomycete that causes wilting and bacterial cancer of tomato (Solanum lycopersicum L.). The Clavibacter genus includes bacteria of economic importance, causing great losses because they infect crops such as corn, alfalfa, potatoes, peppers, tomatoes and wheat and are quarantined worldwide. The objective of this research was to determine the pathogenic capacity of Cmm in different Solanaceae species of economic importance in Mexico. For this reason, inoculations with Cmm were carried out on tomato (Solanum lycopersicum), petunias (Petunia hybrida), potato (Solanum tuberosum), eggplant (Solanum melongena), pepper (Capsicum annuum) and tobacco (Nicotiana tabacum) plants. The TM 68 strain conserved in the strain collection of the Phytopathology laboratory of CIAD, A.C. was used. Culiacán, which was isolated from tomato plants from Ciudad Obregón Sonora, Mexico, and characterized morphologically and molecularly. The results indicate that, in inoculated tomato plants, the presence of symptoms was observed 13 days after inoculation (dai), showing characteristic symptoms such as yellow and rolled leaves, which at 24 dai were dry; In addition, general wilting is observed until finally the plant dies. Regarding the other solanaceous plants, the petunia plants presented symptoms of yellowing at the tips of the leaves and curling at 67 dai. On the other hand, the potato plants showed yellowing at 77 days and the tubers showed a brown ring; and the pepper plants showed strong defoliation. Finally, the eggplant and tobacco plants did not show any symptoms. At 60 days after specific detection of the bacteria using the PCR technique, it was determined positive in the six Solanaceae species. Therefore, it is concluded that the bacterium Clavibacter michiganensis subsp. michiganensis has the ability to reproduce in other solanaceous plants other than tomatoes, although it may or may not cause disease symptoms.