Evaluation of the antimicrobial potential of endophytic microorganisms isolated from Piper hispidinervum

• DOI: 10.22533/at.ed.1593423020110

• Palavras-chave: Piper sp.; Fungal extracts; antimicrobial potential.

• Keywords: Piper sp.; Fungal extracts; antimicrobial potential.

• Abstract:

  Resistance to antimicrobial drugs has become a serious public health problem due to indiscriminate use by humans. Therefore, several alternatives have been studied to contain super microorganisms that cause diseases, among them the use of secondary compounds produced by plants together with endophytic microorganisms. Therefore, this work aimed to isolate, identify and analyze the antimicrobial potential of endophytic microorganisms of Piper hispidinervum C. DC. against pathogens that affect humans and different animals. First, the leaf and inflorescence were collected and the plant material was identified. The material collected went through the process of asepsis and inoculation, which resulted in the growth of endophytes. After their growth, several replications were performed to purify the colonies. Regarding the macromorphology of endophytic fungi, different textures and colors were observed. Were obtained 27 isolates of fungal cultures, among them the most found genera are Colletotrichum sp., and Fusarium sp., we also identified a species of Curvularia sp. The rest of the isolates were classified as Mycelia sterilia. For the antimicrobial susceptibility tests, the inoculum of the test pathogens was carried out in Mueller- Hinton culture medium and the suspension was adjusted to the MacFarland scale. To obtain the fungal extract, colony fragments were deposited in Erlenmeyer flasks with Potato-Dextrose medium, after 14 days the culture medium was filtered, then Ethyl Acetate was added to extract metabolites. The fungal extract obtained by Ethyl Acetate was used in the sensitivity test by the well diffusion method. The Fusarium Extract sp. esp07 was the most effective against the microorganisms tested, being able to inhibit S. aureus, E. coli and C. albicans, resulting in halos with an average of 23 mm, 21 mm and 14 mm, respectively. The Fusarium sp. esp04 and Curvularia sp. fol50 have also been shown to be effective against E. coli.