Antimicrobial activity of silver nanoparticles on biofilm – dental implant model

• DOI: https://doi.org/10.22533/at.ed.1594142426011

• Palavras-chave: Silver nanoparticles. Bacterial biofilm. Dental implants. Dental biomaterials. Streptococcus mutans.

• Keywords: Silver nanoparticles. Bacterial biofilm. Dental implants. Dental biomaterials. Streptococcus mutans.

• Abstract:

  Bacteria are capable of developing biofilms on various types of surfaces, and the bacterial adhesion process can be altered by the characteristics and micromorphology of these surfaces. This way, the properties of biomaterials can be targeted to inhibit bacterial adhesion and colonization. The use of silver is a promising strategy in an attempt to prevent biofilm formation, given its antimicrobial activity. Therefore, the objective of this study was to evaluate the antimicrobial effect of an experimental biomaterial, based on a photopolymerizable orthodontic adhesive (Orthocem UV Trace), modified by the addition of different concentrations of silver nanoparticles (NAg), on biofilm growth (S. mutans). Initially, the surface roughness of the titanium discs, the gap between implant/component and torque/untorque were evaluated. For the biofilm experiment, titanium discs (5 x 2mm) with treated surface (Ti oxide) were used, on which the experimental material was applied, being: G1: Control – biomaterial without addition of NAg; G2: 50ppm; G3: 100ppm; G4: 150ppm; G5: 200ppm; G6: 250ppm. In the end, 2 specimens/group were selected for SEM. The data were not normal, however they were homoscedastic. Thus, post-hoc Tukey (p<0.005) was applied for comparison between groups (Graph 3). The Control group, without the addition of NAg, showed less biofilm growth, while the T200ppm group showed greater growth. The T100 and 150ppm groups were similar to each other, as were the T50 and T250. Considering that the addition of NAg did not present the expected antimicrobial effect and that the reason may have been the unavailability of these on the surface, allowing direct contact with the bacterial biofilm, future research must be conducted, seeking to remedy these difficulties and seeking to highlight the antimicrobial effect of NAg.