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Isolation and Evaluation of Actinomycetes against Phytopathogenic and Bacteria

Microorganisms belonging to the genus Streptomyces are among the major producers of antibiotics, representing more than 50% of the total antimicrobial compounds produced. In this work, the antibacterial activity of 57 species of actinomycetes and 19 bacillary bacteria from different soil samples obtained from different regions of our country was evaluated, where isolation was carried out in potato dextrose medium added with malt and yeast extracts, and then in Petri dish experiments each of the isolated microorganisms were confronted against 2 species of phytopathogenic bacteria and 8 species of bacteria of medical interest. The results obtained indicated that 53% (41) of all isolates showed activity against at least one of the bacteria tested. B. subtilis, B. cereus and S. aureus species were inhibited by a greater number of isolates, while few species showed inhibitory activity against Pseudomonas sp., K. pneumoniae, L. monocytogenes, Salmonella sp. and E. coli (1-4 isolates). The best bacterial inhibition results were obtained on Gram (+) strains; against K. pneumoniae one strain showed 100% inhibition.

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Isolation and Evaluation of Actinomycetes against Phytopathogenic and Bacteria

  • DOI: https://doi.org/10.22533/at.ed.8135125070110

  • Palavras-chave: Actinomycetes, pathogenic bacteria, inhibition, antibacterial activity

  • Keywords: Actinomycetes, pathogenic bacteria, inhibition, antibacterial activity

  • Abstract:

    Microorganisms belonging to the genus Streptomyces are among the major producers of antibiotics, representing more than 50% of the total antimicrobial compounds produced. In this work, the antibacterial activity of 57 species of actinomycetes and 19 bacillary bacteria from different soil samples obtained from different regions of our country was evaluated, where isolation was carried out in potato dextrose medium added with malt and yeast extracts, and then in Petri dish experiments each of the isolated microorganisms were confronted against 2 species of phytopathogenic bacteria and 8 species of bacteria of medical interest. The results obtained indicated that 53% (41) of all isolates showed activity against at least one of the bacteria tested. B. subtilis, B. cereus and S. aureus species were inhibited by a greater number of isolates, while few species showed inhibitory activity against Pseudomonas sp., K. pneumoniae, L. monocytogenes, Salmonella sp. and E. coli (1-4 isolates). The best bacterial inhibition results were obtained on Gram (+) strains; against K. pneumoniae one strain showed 100% inhibition.

  • Maria Guadalupe Maldonado-Blanco
  • Orquídea Pérez González
  • Eduardo Isaí Sauceda Tovar
  • Ma. Guadalupe Rojas Verde
  • Ma. Del Socorro Flores González
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