ANTIOXIDANT ACTION AND CELL VIABILITY OF THE GROSS EXTRACT AND SEED FRACTIONS: Persea Americana mill CULTIVATED IN PIAUÍ

• DOI: 10.22533/at.ed.1592532214097

• Palavras-chave: Antioxidant. Adverse effects. Extract.

• Keywords: Antioxidant. Adverse effects. Extract.

• Abstract:

  Introduction: The compounds that are concentrated in the seed and endocarp are mostly based on compounds of catechins, epicatechins, acids and proanthocyanidins, among others, antioxidants such as vitamins C and E. Goal: To analyze the antioxidant activity and cell viability of the crude extract and the ethyl acetate and aqueous fractions of the fruit fragments: Persea Americana Mill: endocarp with the seed grown in Piauí and list the main substances elucidated in the hydroethanolic extract of these associated parts. Methodology: The hydroethanolic crude extract (7:3) and the H2O:MeOH (7:3) partition were obtained to obtain the ethyl acetate and aqueous fractions. Then, each sample was diluted in 1:1 of MeOH and distilled H2O, to a concentration of 2.0mg/ml. To analyze the antioxidant action, the DPPH (2,2-diphenyl-1-picryl-hydrazyl) test was performed and for cell viability an in vitro colorimetric assay based on the tretazolium salt MTT (3-4,5 dimethylthiazole bromide) was used. -2-yl-2,5-diphenyl tetrazolium) of high efficiency. For the elucidation of the extract molecules: Persea Americana Mill used mass spectrometry (LC-MS, Esquire 2000 Plus, Bruker Daltonics) with a quadrupole ion trap analyzer in tandem mode, with ionization by electronebulization (ESI). Results and discussion: Substances were elucidated whose major compounds were condensed tannins, coming from flavonoids called catechins, specifically, catechin condensates (3.2/289), B1 proanthocyanidinides (3.6/1151), trimers (3.8/863) and catechin tetramers (3.6/1151). The viability level of the crude extract showed levels below 50% at concentrations from 150 µg/ml, indicating high levels of cellular toxicity. However, in the ethyl acetate and aqueous fractions, cell viability did not show worrying levels of toxicity. The analyzed extract also showed good levels of antioxidant action both in the crude extract and in its fractions. Conclusion: The study revealed substances with good antioxidant actions in the investigated extract and that may be beneficial to cardiovascular health. In addition to adding values to the parts discarded by the region of cultivation and subsidizing with information the pharmaceutical and research industries, among other interests.